Table 3: Average brood size following RNAi of selected genes.


Stage Gene N2 (WT) eri-1(-) jun-1 (-) vab-2 (-)

GFP 51.3 ± 9.6 44.8± 4.4 37.5± 6.4 40.3± 5.7
co-located hmg-1.2 47.6± 8.7 1.9± 0.6* 0 34.4± 4.8*
co-located dcp-66 53.7± 8.4 8.3± 0.8** 2.1 ± 0.9* 14.9 ± 1.1
co-located puf-9 48.3± 9.1 37.8±3.9 9.7 ± 3.7* 21.7 ± 4.1*
co-located pas-5 0 0 0 0
co-located let-526 47.8± 5.6 0 0.7 ± 0.3 36.6± 2.2
co-located hpl-2 51.3± 7.3 41.7± 6.1 38.1± 6.6 44.3± 3.6
co-located sop-3 49.6± 8.4 44.6± 4.3 35.9± 5.2 39± 4.2
co-located sor-1 0 0 0 0
co-located bet-1 44.3± 6.6 2.6± 1.3*** 4.6 ± 1.5** 13 ± 2.8*
co-located gei-1 49.9± 11.3 0.7± 0.3 14.3 ± 3.5** 38.9± 5.1
co-located gei-13 5.4± 1.2* 0.7± 0.5 6.1± 1.6 19.3± 3.2
late L3/early L4 gex-3 52.6±7.8 10.9±2.3** 13 ±2.1 17.8 ± 2.9**
mid L4 lin-31 55.9± 5.3 38.6± 3.6 10.6 ±1.3** 39.3±3.7

Average brood size ± SD. N = 15 for each experiment. * = presence of abnormal eggs at ≥ 10% average; ** = presence of abnormal eggs at ≥ 25% average; *** = presence of abnormal eggs at ≥ 50% average. Bolded data indicate synergistic enhancement of brood size phenotype in jun-1 and vab-2 mutants compared to N2.