Figure 3: Binding Isotherms. RNA bound by the N5 sorbent without pre-washing (a) and following the described wash step (b). Here, sorbent masses of 2 (black circle), 8 (red square), 13 (blue triangle), 18 (green diamond), 23 (purple hexagon), and 40 μg (orange star) were utilized for capture of RNA from a 240 μl solution containing ∼300 ng TIM RNA target.