Table 3: Elution solutions and conditions evaluated.


Solution Volume (μL) Time (h) Temperature (C)

EB buffer (10 mM Tris-Cl, pH 8.5) 20, 50, 200 0.3, 3 50
EB buffer (10 mM Tris-Cl, pH 8.5) with 0.1% Tween 20 50 0.3 50
EB buffer (10 mM Tris-Cl, pH 8.5) with 0.1% SDS 50 03 50
EB buffer (10 mM Tris-Cl, pH 8.5) with 0.1% SDS and Tween20 50 0.3 50
Nuclease-free water 20, 50, 200 0.3, 3 RT, 65, 95
1x GoTaq PCR buffer (Promega) with 0.1% SDS 20, 100, 200 0.3, 3 50
NEB buffer 50 0.3 50
NEB buffer with 0.1% SDS 50, 100 0.3 50
50 mM sodium phosphate buffer pH 5.7 to 8.0 50 0.3, 5 50
50 mM sodium phosphate buffer with 0.1% Tween 20, pH7.2 50 0.3 50
10, 50, 100, 200, or 250 mM Tris pH 8.0 50, 100, 200 0.3 50
10, 100, or 200 mM Tris with 20% ethanol 100 0.3 50
10 mM Tris with 50, 100, 150, or 200 mM NaCl pH 8.0 50, 100, 200 0.3 50
10 mM Tris with 1, 5, or 10% Triton X100 pH 8.0 50 0.3 50
10 mM Tris with 100 mM NaCl and 0.1% SDS 100 0.3 50
10 mM Tris with 100 mM NaCl and 1% Triton X-100 100 0.3 50
25 mM Tris with 250 mM Glycine pH 8.0 or 7.0 100 0.3 50
25 mM Tris with 250 mM Glycine & 0.1% SDS pH 8.0 or 7.0 100 0.3 50
25 mM Tris with 250 mM Glycine & 1% Triton X-100 100 0.3 50
100 mM Tris with 50 mM Glycine pH 8.0 or pH 9.5 100 0.3 50
100 mM Tris with 50 mM Glycine and 0.1% SDS pH 9.5 100 0.3 50
100 mM Tris with 50 mM Glycine and 1% Triton X-100 pH 8.0 100 0.3 50
100 or 200 mM Tris with 0.1% SDS pH 8.0 100 0.3 50
200 mM Tris with 50 or 100 mM NaCl pH 8.0 50, 100 0.3 50
200 mM Tris with 100 mM NaCl and 20% ethanol, pH8.0 100 0.3 50
200 mM Tris with 0.1% SDS and 20% ethanol, pH 8.0 100 0.3 50
200 mM Tris with 0.1% SDS, pH 8.0 100 0.3 50
Hyb buffer (MiSeq) 100 0.3 50
Hyb buffer (Affymetrix) 100 0.3 50
0.2X or 2X SSC with 0.1% SDS 100 0.3 50
0.6X or 6X SSPE with 0.1% SDS 100 0.3 50
0.31, 0.63, 1.3, or 2.5 M NaCl 100 0.3 50
50 mM Glycine with 150 mM NaCl pH 9.5 100 0.3 50
50 mM Glycine with 150 mM NaCl and 0.1% SDS pH 9.5 100 0.3 50
1xTAE with 0.1%SDS 100 0.3 50
Washed Sorbents
10 mM Tris pH 8.0 100 0.3 50
10 mM Tris with 20% ethanol 100 0.3 50
100 mM Tris with 0.1% SDS 100 0.3 50
10 mM Tris with 100 mM NaCl 100 0.3 50
NEB with 0.1% SDS 100 0.3 50
Washed DEN Sorbent
1x PCR 0.1% SDS 100 0.3 50
NEB with 0.1% SDS
10 mM Tris pH 8.0 100 0.3 50
10 mM Tris with 20% ethanol 100 0.3 50
10 mM Tris with 100 mM NaCl 100 0.3 50
10 mM Tris with 100 mM NaCl and 0.1% SDS 100 0.3 50
10 mM Tris with 100 mM NaCl and 1% Triton X-100 100 0.3 50
25 mM Tris with 250 mM Glycine and 0.1% SDS 100 0.3 50
25 mM Tris with 250 mM Glycine and 1% Triton X-100 100 0.3 50
100 mM Tris with 0.1% SDS 100 0.3 50
100 mM Tris with 50 mM Glycine and 0.1% SDS 100 0.3 50
100 mM Tris with 50 mM Glycine and 1% Triton X-100 100 0.3 50
0.6X SSPE with 0.1% SDS 100 0.3 50
0.6X SSPE with 1% Triton X-100 100 0.3 50