Abstract
Author(s): Laureana Stelmastchuk Benassi Fontolan
The production of recombinant proteins for therapeutics, veterinary and agricultural purposes is a very successful technology. The process relies on achieving theproper cell growth conditions and induction of protein expression, which requires a careful screening experiment before scaling up the production. Automation in a miniaturized and high throughput format for protein production allows researchers to conduct higher complexity experiments as it handles a number of different samples and conditions simultaneously. For structural biology studies, obtaining well expressing and correctly folded protein from gene constructs is still a time consuming process.nAlthough various methods are available, the equipment required for parallel expression experiments is expensive and restricted to facilities operated by trained personnel. This study reports the construction of an inexpensive customizable device for high throughput protein expressionmaking use of 3D printing techniques and free software/ hardware design (1,2).
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